Table 2.

Details of the chosen domains of PhtD, PsaA and PspC for designing the final construct

Protein	PhtD-C	PsaA	PspC
Final selected domain	(Residues 196–256) AAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGS	(Residues 100–187) SDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSAKDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEG	(Residues 276–363) RRNYPSNTYFSLELEISESDVEVKKAEFELVKEEAKEPRNEEKVKQAKAKVESKKAVATRLENIKTDRKKAEEEAKRKAAEEDKVKEK
Final B-cell epitopes	224–238 225–239 226–240 234–248 237–251 238–252 239–253 240–255 241–256	103–117 104–118 105–119 106–120 107–121 108–122 109–123 110–124 113–127 133–147 141–155 148–162	285–299 288–302 294–308 295–309 324–339 327–341
Final MHC-II Epitopes (Mouse)	189–203 190–204 217–231 218–232 223–237 224–238 225–239 226–240 227–241	174–188 175–199 176–190	288–302 289–303 290–304 298–312 299–313 300–314 301–315 316–330 333–347 323–337 322–336 321–335 324–338 320–334
Final MHC-II epitopes (Human)	222–236 223–237 224–238 225–239 227–241 228–242 240–254 241–255	103–117 104–118 126–140 167–181 168–182 169–183	303–317 334–348 335–349 336–350 337–351 333–347