Fig. 7.

ITGA5 can be O-glycosylated by GALNT2 in NSCLC. A Functional pathway analysis by GSEA. B VVA binding assay for the detection of O-linked glycosylation. C Heatmap displaying the Pearson correlation between GALNT2 and the Integrin pathway-related genes. D Detection of ITGA5 O-glycosylation by pull-down assay. E Western blot analysis of ITGA5 expression following siRNA transfection. F CCK-8 assay. G Colony formation assay. H Transwell migration and Matrigel invasion assays (magnification, ×100). I Protein–protein interaction network. OV GALNT2 overexpression lentivirus, Mock normal control lentivirus, shRNA GALNT2 shRNA lentivirus, shNC negative control shRNA lentivirus, siRNA ITGA5 siRNA, siNC negative control siRNA, NS no significance; *P < 0.05; **P < 0.01; ***P < 0.001