Fig. 9.

miR-30d negatively regulates GALNT2 expression in NSCLC. A Four databases were employed to predict the potential miRNAs targeting GALNT2. B Heatmaps of the top 50 miRNAs negatively associated with GALNT2 in NSCLC. C Analysis of miR-30d expression in TCGA. D Analysis of miR-30d expression using cDNA microarrays. E Detection of GALNT2 expression by RT-qPCR. F Detection of GALNT2 expression by Western blot. G Schematic representation of the putative binding site for miR-30d in the GALNT2 3′-UTR. H Luciferase reporter assay. I CCK-8 assay. J Colony formation assay. K Transwell migration and Matrigel invasion assays (magnification, ×100). OV GALNT2 overexpression lentivirus. ^#P > 0.05, *P < 0.05