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. 2022 Sep 3;13(9):763. doi: 10.1038/s41419-022-05208-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Flowchart of IPA analysis and the top 5 significant pathways of DEGs in integrated analysis of LC-MS/MS and RNA-Seq data. B, C The mRNA levels of DEGs enriched in ‘glycolysis’ were validated using reverse transcription-quantitative PCR in SMYD3-knockdown OCI-LY8 cells and NC cells and were visualized in the heatmap. (The names of the different columns in the heatmap were shown). D The mRNA and protein expression of PKM2 decreased following SMYD3knockdown in the OCI-LY1 and OCI-LY8 cell lines. E SMYD3 and PKM2 staining in xenograft tissue samples from mice injected with SMYD3-knockdown OCI-LY8 and NC cells. Magnification, x200. Scale bar,100 μm. F Glucose uptake, cellular pyruvate levels, lactate production and G ECAR significantly decreased following SMYD3 knockdown in the OCI-LY1 and OCI-LY8 cell lines. H SMYD3 knockdown inhibited the glucose uptake of DLBCLs in vivo. I Illustration of SUVmax from PET-CT scans of patients with DLBCL and high or low SMYD3 protein expression. J High SMYD3 protein expression was significantly associated with the SUVmax of patients with DLBCL. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. DLBCL Diffuse large B-cell lymphoma, SMYD3 SET and MYND domain containing 3, PKM2 Pyruvate kinase M2, DEG Differentially expressed gene, IPA Ingenuity pathway analysis, LC Liquid chromatography, MS Mass spectrometry, RNA-Seq RNA sequencing, NC Negative control, PET Positron emission tomography, CT Computed tomography, ECAR Extracellular acidification rate, SUVmax Maximum standardized uptake value.