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. 2022 Sep 3;13:5202. doi: 10.1038/s41467-022-32788-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic showing administration protocol for spermidine (3 mM in drinking water) in mice fed with WDF for 16 weeks. b Representative images of H&E staining of the liver. (n = 5). c Hepatic triglyceride content was measured by biochemical assay. (n = 8). d–g Liquid chromatography coupled with mass spectrometry (LC-MS) lipidomic profiling of hepatic triacylglycerols (TAGs) (d), diacylclycerols (DAGs) (e), monoacylglycerol (MAGs) (f), and cholesterol esters (CEs) (g) levels from mice fed with NCD (n = 6), WDF (n = 8), or WDF supplemented with spermidine (WDF + Spd, n = 6) for 16 weeks. Heat maps represent the average of each condition. h circulating ALT level from mice fed with NCD (n = 8), WDF (n = 8), WDF + Spd (n = 7). i Hepatic collagen content was determined by measurement of hydroxyproline level in the liver from mice fed with NCD (n = 6), WDF (n = 8), WDF + Spd (n = 7). j Representative images of Masson trichrome stain of liver sections. (n = 5). Significance was calculated by One-Way ANOVA. Source data are provided as a Source Data file.