Fig. 3.

Generation and characterization of bead-immobilized PA-DDAH. PA-DDAH was dialyzed against 0.1 M phosphate, pH 8. Indicated concentrations in 500 μL buffer were incubated with 20 mg of agarose-NHS beads for 2 h at room temperature, while mixing. The conjugated beads were washed and assayed for DDAH activity. (a) Concentration-dependence of DDAH conjugation to the agarose beads (n=3). (b) Shows that the activity of the enzyme was fully retained after conjugation, activity prior to conjugation and (●) and after conjugated (■) (n=3). Stability of the immobilized PA-DDAH was determined after storage of the beads for up to one month at 4 ˚C (n=3). All data are given as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.