TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | F− Δ(lacZYA-algF)U169 thi-1 hsdR17 gyrA96 recA1 endA1 supE44 relA1 phoA φ80 dlacZΔM15 | 20 |
| CC118(λpir) | Δ(ara-leu) araD ΔlacZ74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1, λpir | 21 |
| P. aeruginosa strains | ||
| 7NSK2 | Wild type | 22 |
| PAK | Wild type | 4 |
| PAO1 | Wild type | 23 |
| PAO25 | PAO1 leu arg | 18 |
| PAN1 | PAO25 xcpQ::Gmr | 3 |
| PAN8 | PAO25 lasB::KmraprE | 6 |
| PAN9 | PAO25 xcpQ::GmrlasB::KmraprE::Hgr | 6 |
| PAN10 | PAO25 lasB::Kmr | 6 |
| PAN17 | PAO25 cbpD::Kmr | This study |
| PAN101-124 | Clinical isolates of P. aeruginosa | This study |
| P. fluorescens strains | ||
| WCS374 | Wild type | 16 |
| WCS417 | Wild type | 31 |
| En401 | Wild type | 48 |
| RS111 | Wild type | 48 |
| P. putida WCS358 | Wild type | 16 |
| S. aureus | Wild type | Laboratory strain |
| Plasmids | ||
| pBSL99 | AmpR, KmR, kanamycin-resistance cassette | 1 |
| pKNG101 | SmR, oriR6K, mobRK2, sacBR | 25 |
| pMMB67EH | AmpR, RSF replicon (IncQ), tac promoter | 14 |
| pRK2013 | KmR, Tra+, Mob+ | 10 |
| pUC18 | AmpR, ColE1, φ80dlacZ, lac promoter | 35 |
| pUC19 | AmpR, ColE1, φ80dlacZ, lac promoter | 52 |
| pJF29 | AmpR, cbpD gene in pUC19 opposite orientation relative to Plac | This study |
| pJF30 | AmpR, KmR kanamycin-resistance cassette insertion in cbpD of pJF29 | This study |
| pJF31 | KmR, SmR, cbpD::Kmr in pJF30 in pKNG101 | This study |
| pJF38 | AmpR, cbpD gene in pMMB67EH | This study |
a
Abbreviations: Amp, ampicillin; Gm, gentamycin; Hg, mercury; Km, kanamycin; Sm, streptomycin.