Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 4;28:102. doi: 10.1186/s10020-022-00530-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Macrophages were actively involved in inflammatory bone remodeling and M1 macrophages were the major activated macrophage subtype in OA bone remodeling areas. A(i) H&E, TRAP, and IHC staining of macrophage specific markers (CD68: macrophage pan marker; iNOS: M1 macrophage marker; CD206: M2 macrophage marker; positive cells were labeled with red arrows) on normal and OA bone sections (a and f: scale bars represented was 200 μm in H&E staining; b and g: scale bars represented was 20 μm in TRAP staining; c, d, e, h, i, and j: scale bars represented was 20 μm in IHC staining); A(ii): The box plot graphs demonstrated the positive cells per field of view. Data was shown as the mean ± SD (*p < 0.05, t-test); B IF double staining of macrophage markers on normal and OA bone sections (CD68 and CD11b: macrophage pan markers; CD86: M1 macrophage marker, CD206: M2 macrophage marker; scale bars represented 20 μm); C IF double staining of M1 macrophage marker and osteocyte marker on OA bone sections (CD86: M1 macrophage marker; E11: early osteocyte marker; DMP1: mature osteocyte marker; scale bars represented 20 μm)