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Northern analysis of fabF expression in P. profundum strains EA40 (fabF) and DB110 (wild type). Cells were grown to late exponential phase for total RNA isolation and probed using internal fragment of SS9 fabF as described in Materials and Methods. Lanes: 1, EA40 at 4°C; 2, EA40 at 15°C; 3, DB110 at 4°C; 4, DB110 at 15°C; 5, DB110 at 28 MPa (9°C); 6, DB110 at 0.1 MPa (9°C).
