TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| Photobacterium profundum | ||
| SS9 | Wild type | 11 |
| DB110 | Lac− Rifr; SS9 derivative | 8 |
| EA40 | fabF disruption mutant; DB110 derivative | This study |
| E. coli | ||
| SJ16 | panD2 zad-220::Tn10 | 24 |
| MR86 | fabF::Kmr insertion; SJ16 derivative | 31 |
| MG1655 | λ− | 21 |
| 1100 | λ−glnV44 rfbD1 endA1 spoT1 thi-1 | 15 |
| Plasmids | ||
| pCR2.1 | Vector for cloning PCR products, Kmr | Invitrogen |
| pEA39 | 740-bp internal fragment of SS9 fabF in pCR2.1, Kmr | This study |
| pMUT100 | Mobilizable suicide plasmid, Kmr | 7 |
| pEA40 | 740-bp internal fragment of SS9 fabF in pMUT100, Kmr | This study |
| pEA401 | SS9 genomic library clone containing fabF and flanking DNA, Kmr | This study |
| pKT231 | Broad-host-range vector, Kmr Smr | 3 |
| pEA44 | SS9 fabF in pKT231, Smr | This study |