TABLE 2.
Fatty acid composition of P. profundum strains as a function of varying temperature and pressurea
| Fatty acid species | Mean wt% fatty acid species ± SD
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Wild type
|
fabF
|
|||||||
| Temp (°C)
|
Pressure (MPa)
|
Temp (°C)
|
Pressure (MPa)
|
|||||
| 15 | 4 | 0.1 MPa | 28 | 15 | 4 | 0.1 MPa | 28 | |
| 12:0 | 1.8 ± 0.9 | 2.7 ± 1.0 | 1.5 ± 0.1 | 2.2 ± 0.6 | 3.2 ± 0.5 | 4.3 ± 1.4 | 4.0 ± 1.3 | 3.4 ± 0.6 |
| 14:0 | 4.9 ± 1.4 | 3.3 ± 0.8 | 5.0 ± 0.3 | 1.6 ± 0.7 | 13.2 ± 1.7 | 18.6 ± 3.2 | 19.6 ± 1.3 | 14.0 ± 1.8 |
| 14:1 | 1.2 ± 0.8 | 2.5 ± 0.5 | 0.8 ± 0.1 | 0.7 ± 0.1 | 4.6 ± 1.1 | 6.3 ± 0.8 | 4.8 ± 0.9 | 3.1 ± 0.8 |
| Iso-16:0 | 9.8 ± 3.1 | 0.2 ± 0.1 | 1.2 ± 1.3 | |||||
| 16:0 | 21.7 ± 2.8 | 20.2 ± 3.8 | 21.4 ± 1.5 | 17.5 ± 3.2 | 19.2 ± 2.6 | 10.5 ± 2.2 | 19.7 ± 1.3 | 16.2 ± 1.2 |
| 16:1 | 41.3 ± 8.3 | 48.8 ± 6.8 | 64.1 ± 1.5 | 48.7 ± 9.6 | 41.5 ± 4.8 | 39.3 ± 6.8 | 31.4 ± 1.1 | 44.9 ± 0.7 |
| 12-OH | 3.0 ± 1.2 | 3.4 ± 1.3 | 0.6 ± 0.1 | 2.0 ± 0.8 | 3.8 ± 1.0 | 4.1 ± 1.2 | 3.4 ± 1.2 | 5.2 ± 0.1 |
| 18:0 | 1.1 ± 0.4 | 0.2 ± 0.1 | 0.2 ± 0.0 | 0.1 ± 0.1 | 1.9 ± 0.4 | 0.1 ± 0.0 | 0.7 ± 0.1 | 0.4 ± 0.1 |
| 18:1 | 9.9 ± 2.6 | 10.3 ± 1.7 | 3.6 ± 0.1 | 16.2 ± 1.6 | 4.0 ± 0.8 | 0.7 ± 0.1 | 1.1 ± 0.3 | 1.1 ± 0.3 |
| 20:5 | 5.3 ± 1.1 | 8.4 ± 1.1 | 2.7 ± 0.1 | 11.0 ± 0.8 | 7.1 ± 1.7 | 15.9 ± 2.1 | 15.1 ± 3.8 | 11.4 ± 1.5 |
| UFA/SFA ratiob | 1.95 | 2.65 | 2.53 | 3.57 | 1.53 | 1.91 | 1.20 | 1.77 |
a
Data represent values derived from triplicate samples harvested in late-exponential-phase growth; 15 and 4°C cultures were grown aerobically at 0.1 MPa; 0.1- and 28-MPa cultures were grown at 9°C in 2216 marine medium containing 22 mM glucose buffered with 100 mM HEPES.
b
UFAs, 14:1, 16:1, 18:1, and 20:5; SFAs, 12:0, 14:0, 16:0, and 18:0.