Figure 2.

Dose–response curves of AZD0449 and AZD4604 showing mean (A) inhibition of IL-4-induced phosphorylation of STAT6 in human T cells from PBMCs, (B) inhibition of IL-2-induced phosphorylation of STAT5 in human and rat T cells from PBMCs, and (C) inhibition of IFNα- and IL-12-induced phosphorylation of STAT1 and STAT4 in human T cells and NK cells expanded from PBMCs. All assessments were made by intracellular immunofluorescent labeling and flow cytometry. In (B), owing to the discontinuation of the rat pSTAT6 antibody, pSTAT5 was evaluated as a readout. In (C), pSTAT1 inhibition was determined in human cells gated as monocytes and pSTAT4 was determined in human cells gated as T cells or NK cells. Error bars represent SD. IC₅₀s were not adjusted for protein binding.

Abbreviations: IFN, interferon; IL, interleukin; n, number of biological replicates; NK, natural killer; pIC₅₀, phosphorylated half maximal inhibitory concentration; PBMC, peripheral blood mononuclear cell; pSTAT, phosphorylated signal transducer and activator of transcription; SD, standard deviation.