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Time course of Pa-MIG glycosylase activity on substrates T/G, T/GO, U/G, and U/GO. Five nanograms of Pa-MIG was incubated with 96-bp heteroduplex DNA containing T/G, T/GO, U/G, or U/GO mismatches. The uncut 96-mer DNA substrate (S) and cleaved 60-mer product (P) are indicated on the left. Asterisks indicate the 5′-end-labeled strand.
