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. 2022 Jul 25;226(4):566–575. doi: 10.1093/infdis/jiac307

Figure 2.

Figure 2

NK cells from cCMV-positive neonates express higher levels of cytotoxic mediators. A, Frequency of cord blood NK cells expressing granzyme B, perforin, and granulysin from cCMV-positive newborns (n = 16) and cCMV-negative controls (n = 69). Granulysin expression was evaluated on a smaller subset of cord blood samples (cCMV-positive, n = 8; cCMV-negative, n = 36). NK cells are defined as lymphocytes > single cells >CD3−/CD14−/CD19− > CD56+ and/or CD16+. Note that CD7 was not included in the flow cytometry panel used to evaluate markers listed in Figure 2. See details in Supplementary Figure 2B. B, Density of granzyme B, perforin, and granulysin expression on NK-cell subsets measured as normalized MFI from cCMV-positive newborns (n = 16) and cCMV-negative controls (n = 69). C, Proportion of NK-cell subsets expressing the listed combination of granzyme B and perforin in cCMV-negative (n = 69) and cCMV-positive infants (n = 16). Coexpression was calculated using Boolean gating in FlowJo and pie graphs, depicting average proportions, were generated in SPICE. D, Frequency of NK cells expressing T-bet, eomesodermin, and Ki67 in cCMV-positive infants (n = 16) and cCMV-negative controls (n = 69). *P < .05, **P < .01, ***P < .001, Wilcoxon rank sum test. Abbreviations: cCMV, congenital cytomegalovirus; GzB, granzyme B; MFI, mean fluorescence intensity; NK cell, natural killer cell; Pfn, perforin.