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. Author manuscript; available in PMC: 2022 Sep 4.
Published in final edited form as: Arch Toxicol. 2019 Feb 27;93(4):1051–1065. doi: 10.1007/s00204-019-02405-w

Figure 7. Over-expression of calpain-2 reduces PTEN protein levels and increases Akt activation and MKP-1 protein expression in Tg-Capn2/tTA mouse hearts.

Figure 7.

Tg-Capn2/tTA (TG) and Tg-Capn2 mice (WT) received doxorubicin or saline. Five days later, heart tissues were analyzed for PTEN, Akt and MKP-1 protein expression. (a) Upper panel; representative western blot for PTEN and GAPDH from 2 out of 5 different hearts from WT and TG at 5 days, lower panel: quantitation of MKP-1/GAPDH ratio. (b) Upper panel; representative western blot for Akt and phosphorylated Akt (Ser308 and Thr473) from 2 out of 6 different hearts form WT and TG, lower panel; quantitation of phosphorylated Akt/Akt ratio. (c) Upper panel; representative western blot for MKP-1 and GAPDH from 2 out of 5 different hearts from WT and TG, lower panel: quantitation of MKP-1/GAPDH ratio. Data are mean ± SD, n=5-6 different hearts. *P < 0.05 versus WT + saline and †P < 0.05 versus WT + Doxorubicin.