Table 1.
Mass Spectrometry Settings
Instrument-dependent parameters | ||||||
---|---|---|---|---|---|---|
Ion Spray Voltage (V) | Temperature (°C) | Collision gas | Curtain gas (psi) | Ion source gas 1 (psi) | Ion source gas 2 (psi) | |
5500 | 600 | High | 20 | 30 | 50 | |
Compound-dependent parameters for analytes | ||||||
Analyte | Q1 (m/z) | Q3 (m/z) | DP (V) | EP (V) | CE (V) | CXP (V) |
VCR | 825.4 | 807.6 | 150 | 7 | 57 | 9 |
M1 metabolite | 397.3 | 337.4 | 70 | 7 | 32 | 6 |
VRL | 390.3 | 122.1 | 50 | 3 | 18 | 6 |
Parent ion (Q1), Product ion (Q3), declustering potential (DP), entrance potential (EP), collision energy (CE) and cell exit potential (CXP) for VCR, M1, and VRL, respectively.