TABLE 2.

Metabolic reprogramming profiling by omics- analysis in OS.

Non-metastatic model		Metastatic model		References
Cell line	Characteristics	Cell line	Characteristics
HOSMMNG/HOS	Lower expression of TCA cycle and amino acid -related enzymes and metabolites than malignant MNNG/HOS and 143B cell lines	143B	Higher expression of glycolytic metabolites and glycolysis-involved enzymes with a non-significant difference between MNNG/HOS and 143B cell lines	Fritsche-Guenther et al. (2020)
	Lower expression of lipid compounds than MNNG/HOS and 143B cell line		Lower expression of TCA cycle and amino acid -related enzymes and metabolites than malignant MNNG/HOS cell line but higher than HOS cell line
	Reduced viability in glutamine-deprived conditions		Lower expression of lipid compounds than MNNG/HOS cell line but higher than HOS cell line
			Metabolic flexibility in using either glucose or glutamine for its proliferation
HOS	Lower expression of phosphatidylethanolamines, phosphatidylcholines, lysophosphatidylcholines, and ceramides than non-cancerous hFOB cell line	143B	Lower expression of phosphatidylethanolamines, phosphatidylcholines, lysophosphatidylcholines, and ceramides than non-cancerous hFOB cell line	Roy et al. (2019)
	Upregulation of cardiolipins		Higher expression of monoacylglycerol and diacylglycerol than hFOB and HOS cell lines
			Lowest expression of lysophosphatidylcholine, lysophosphatidylethanolamine, phosphatidylglycerol, triacylglycerol, cholesteryl esters
3 weeks of LM8 inoculation in mice (starting point of metastasis)		4 weeks of LM8 inoculation in mice (lung metastasis)
Increases of glycolysis, TCA cycle-related metabolites, 2-HG, succinic acid, and most of amino acids, including serine and methionine		Decrease of carbohydrate and amino acid metabolism		Hua et al. (2011)
		Increase of fatty acid metabolism