TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| R. leguminosarum bv. viciae isogenic strains | ||
| 8401 | R. leguminosarum lacking pSym, Strr | 28 |
| 8401/pRL1JI | Derivative of 8401 carrying the symbiotic plasmid pRL1JI | 12 |
| A168 | 8401/pRL1JI pssA1::Tn5 | 4 |
| A412 | 8401/pRL1JI prsD1::Tn5 | 14 |
| A507 | 8401/pRL1JI pss5::Tn5 | This work |
| A517 | 8401/pRL1JI pss6::Tn5 | This work |
| A550 | 8401/pRL1JI ΔplyA-prs-pss::nptII | This work |
| A600 | 8401/pRL1JI plyB1::Tn5 | 15 |
| A638 | 8401/pRL1JI plyA3::Spcr | 15 |
| A640 | 8401/pRL1JI plyA3::SpcrplyB1::Tn5 | 15 |
| Other strains | ||
| 1021 | R. meliloti | S. Long |
| 3855 | R. leguminosarum bv. viciae | 25 |
| ANU843 | R. leguminosarum bv. trifolii | 9 |
| ANU2840 | Tn5-induced EPS− mutant of ANU280 (Smr Rifr derivative of NGR234) | 6 |
| C58C1 | A. tumefaciens | 47 |
| CE3 | R. etli | 35 |
| CIAT899 | R. tropici | 32 |
| NZP2213 | R. loti | DSIR Culture Collection |
| RCR5 | R. leguminosarum bv. trifolii | 22 |
| Rhizobium sp. strain NGR234 | Broad-host-range Rhizobium sp. | 44 |
| USDA193 | S. fredii | USDA-ARS National Rhizobium Culture Collection |
| VF39 | R. leguminosarum bv. viciae | 37 |
| Plasmids | ||
| pIJ7298 | pLAFR1 cosmid carrying prsDE and plyA | 14 |
| pIJ7471 | HindIII deletion of pIJ7298 with the kanamycin resistance gene from Tn5 on a 3.4-kb HindIII fragment | This work |
| pIJ7709 | plyB cloned in pIJ1891 | 15 |
| pIJ7871 | plyA cloned behind a vector promoter in pKT230 | 15 |
| pRGC1 | pLAFR1 cosmid carrying the egl endoglycanase gene from A. caulinodans | 17 |