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. 2022 Aug 22;13:938596. doi: 10.3389/fendo.2022.938596

Figure 1.

Figure 1

Effect of TH on Purkinje cell dendrite arborization in primary cerebellar culture. Cerebellar cells were cultured for 17 days, followed by an immunocytochemistry analysis of Purkinje cells with Calbindin D-28K (green) and DAPI (blue). (A) Representative photomicrographs showing the effects of TH on the morphology of Purkinje cells. (B) Schematic diagram of Sholl analysis. (C) Quantitative analysis of the effect of TH on the number of dendrite intersections at each concentric circle by Sholl analysis. (D) Quantitative analysis of the effect of TH on the number of secondary and tertiary dendrites of Cerebellar Purkinje cells. (E) Changes in the dendritic areas of Purkinje cells following TH treatment. ImageJ Fiji software (NIH) was used to quantify the dendritic area of Purkinje cells and Sholl analysis. Bars indicate 50 μm. Data are shown as the mean ± SEM and representative from at least three independent experiments. ****p < 0.0001 and **p < 0.001 indicate statistical significance according to two-way or one-way ANOVA continued with post hoc Turkey test. See Supplementary Table 1 .