Figure 2.

TR deletion impaired TH-augmented dendrite arborization of Purkinje cells. Cerebellar cells were cultured for 17 days, followed by an immunocytochemistry analysis with Calbindin D-28K (green). (A) Representative photomicrographs showing the effects of TH on the morphology of Purkinje cells after TR knockdown. Quantitative analysis of the effect of TH after exposure to TRα siRNA (B), TRβ siRNA (C), and siRNA against TRα and TRβ (D) on dendritic areas of Purkinje cells following TH treatment. ImageJ Fiji software (NIH) was used to quantify the dendritic area of Purkinje cells. Bars indicate 50 μm. Data are shown as the mean ± SEM and representative from at least three independent experiments. ****p < 0.0001 indicates statistical significance according to two-way or one-way ANOVA continued with post hoc Turkey test. See Supplementary Table 1 .