Figure 4.

TRα and integrin αvβ3 affect neurite outgrowth induced by TH in neuronal-derived Neuro-2A cells. Cells were induced to differentiate by serum starvation for one to three days, followed by immunofluorescence analysis with β-tubulin III (red), doublecortin (green), and DAPI (blue). (A) Representative photomicrographs showing the effects of TH on the differentiation of Neuro-2A cells. (B) Changes in neurite lengths after TH exposure. (C) Quantitative analysis of the effect of TH on the number of neurite intersections at each concentric circle by Sholl analysis. Neurite length and Sholl analysis were quantified using ImageJ Fiji software (NIH). Bars indicate 50 μm. Quantitative analysis of the effect of TH after exposure to TRa siRNA (D), TRb siRNA (E), TRa and TRb siRNA (F), and LM609 2 μg/mL (G) on neurite length in Neuro-2A cells. ****p < 0.0001, indicates statistical significance according to two-way or one-way ANOVA continued with post hoc Turkey test compared to control. ^#### p < 0.0001 and ^## p < 0.01, indicate statistical significance according to two-way ANOVA continued with post hoc Bonferroni’s test. See Supplementary Table 1 .