FIG. 4.

Mapping of the parAB transcription start points and detection of the parAB transcript level during development in surface-grown culture of the wild-type S. coelicolor strain M145. S1 mapping of parAB mRNA was carried out with RNA isolated from M145 grown on MM containing mannitol for different periods (18, 24, 36, 48, and 72 h). The presence of vegetative mycelium, aerial mycelium, and spores at each time of harvest was judged by microscopic examination. The first and second panels were from the same gel, and thus the proportion of two transcripts is valid for comparison. The sequence of the template strand is shown on the right, and the two transcription start points are indicated by asterisks. The levels of hrdB mRNA in the same RNA samples were measured by S1 mapping as an internal control.