Figure 2.
The contribution of fucose salvage into N-glycans is almost insensitive to exogenous fucose. (A and C) Incorporation of 5 mM 13C-1,2-glucose, 50 μM 13C-1,2,3-mannose and 13C-6-fucose into fucose associated with newly synthesized N-glycoproteins secreted by HepG2, Huh7, and CHO cells pre-labeled for 11 d with 50 μM 13C-UL-fucose expressed as a percentage of labeling (A) and as a percentage of maximal signal (C); n = 3; data are presented as mean ± SD. (B) Schematic representation of the results are presented in A.