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. 2022 Mar 3;42(9):1650–1665. doi: 10.1177/0271678X221080324

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© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Granule cells from Sprague-Dawley rat decrease the sEPSC and firing frequency after activation of HCAR1. (a) Example traces of sEPSCs measure during baseline condition (black) and following application of 40 µM 3Cl-HBA (red). (b) Cumulative distributions of sEPSCs frequency were analyzed by using Kolmogorov-Smirnov test: P < 0.0001 versus HCAR1 activation. (c) Summary of recorded cells showing a decreased frequency of sEPSC by 27% induced by 3Cl-HBA compared to baseline (one sample t, n = 13, mean = 72.77 ± 35.96, P = 0.018). (d) sEPSC amplitude showed no statistically significant changes between baseline and 3Cl-HBA application (one sample t, n = 13, mean = 103.2 ± 18.38, P = 0.0049). Frequency and amplitude are shown in percentage compared to baseline. (e) Summary graph of the effect of HCAR1 activation on neuronal firing frequency following steps of current injection. The maximum number of action potentials evoked was calculated for each condition. No difference was observed (paired t test, n = 7, Baseline: mean = 51.69 ± 16.79, 3Cl-HBA: mean = 47 ± 11.59, P = 0.13). (f) The current injected to reach the maximum firing frequency was calculated for each condition. HCAR1 activation significantly decreases the current needed for reaching the maximum firing frequency (paired t test, n = 13, Baseline: mean = 327.7 ± 96.79, 3Cl-HBA: mean = 270 ± 80.31, P = 0.0049). Values are means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 versus 3Cl-HBA application.