FIG. 1.

Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila. PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.