TABLE 4.
Ptools pathway analysis results for 59 selected DEGs responsive to 2,4-D treatment in the herbicide-tolerant cotton line CS-B15sh showing the identified pathways and reactions for five DEGs.
| DEG | Description | Pathway description | BioCyc pathway ID | Reaction description | BioCyc reaction ID |
| Gohir.A12G201300.1; Gohir.D08G169900.1 | Cytochrome P450, E-class, group I | Abscisic acid degradation to phaseic acid | PWY-5271 | 2-cis-abscisate + a reduced [NADPH-hemoprotein reductase] + oxygen → 8′-hydroxyabscisate + an oxidized [NADPH-hemoprotein reductase] + H2O | 1.14.13.93-RXN |
| Gohir.D05G079500.1 | Unknown function | Abscisic acid biosynthesis | PWY-695 | (+)-cis-abscisic aldehyde + H2O + oxygen → 2-cis-abscisate + hydrogen peroxide + H+ | 1.2.3.14-RXN |
| Gohir.A13G049200.1; Gohir.A13G049200.2 | Amidase signature (AS) superfamily | L-arginine degradation × (arginine monooxygenase pathway) | ARGDEG-V-PWY | A monocarboxylic acid amide + H2O → a monocarboxylate + ammonium | AMIDASE-RXN |
| 4-guanidinobutyramide + H2O → ammonium + 4-guanidinobutanoate | GUANIDINOBUTANA MIDE-NH3-RXN | ||||
| 2-hydroxyisobutyramide + H2O → 2-hydroxy-2-methylpropanoate + ammonium | RXN-17608 | ||||
| Acetamide + H2O → acetate + ammonium | RXN-14728 | ||||
| Propionamide + H2O → propanoate + ammonium | RXN-14727 | ||||
| Acrylamide + H2O → ammonium + acrylate | R311-RXN | ||||
| (Indol-3-yl)acetamide + H2O → (indol-3-yl)acetate + ammonium | RXNN-404 | ||||
| Pyrazinamide + H2O → ammonium + pyrazine-2-carboxylate | PYRAZIN-RXN | ||||
| Gohir.D11G154700.1 | Zinc finger, RING-type | Protein ubiquitination | PWY-7511 | An [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + a [protein]-L-lysine → an [E2 ubiquitin-conjugating enzyme]- L-cysteine + a [protein]-N6-monoubiquitinyl-L-lysine + H+ | RXN-15561 |