Figure 5.

Hic2 is a possible gene target of miR-6068 in miR-mimic-transfected PCa cells. A: Selected top listed target genes of miR-6068 using three different prediction bioinformatic algorithms explaining the binding site of miR-6068 on the 3’-UTR of hic2 gene. B: Transient transfection of PCa cells with miR-6068 downregulates the hic2 gene. C: Western blot analysis (Left) shows a decrease in HIC2 and SIRT1 after transfection of PCa cells with miR-6068. D: The quantification of HIC2 and SIRT1 protein expression was performed. *Significance was calculated at P<0.05. The experiments were repeated at least twice.