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. 2022 Aug 15;12(8):3693–3712.

Figure 4.

Figure 4

Ku80 knockdown and inhibition of LSD1 repress invasiveness and proliferation and induces apoptosis of colon cancer cells in vitro. A. Western blot detected the shRNA transfection efficiency. B. Statistically significant differences among Different interference sequences. C. Effect of different concentrations of RN-1 on colon cancer HCT116 cell growth. D. At 24 h, 48 h, and 72 h, the proliferation activity of HCT116 cells in the experimental group (shKu80#2, RN-1 group, shKu80#2 + RN-1 group) was significantly inhibited compared with the control group. At 24 h, 48 h, and 72 h, there was no significant difference in the proliferation activity of HCT116 cells in the shKu80#2 and RN-1 groups, but the shKu80#2 + RN-1 group had significant differences compared with the shKu80#2 and RN-1 groups, respectively. E, F. Edu assay also obtained similar results. G-N. Effect of HCT116 cells that transfected shRNA#2 or/and RN-1-treated was observed on the invasion, migration, apoptosis, and clone formation. O-Q. The expression of LSD1, Ku80, and FOXF2 in HCT116 cells that transfected shRNA#2 or/and RN-1-treated were detected by Western blot. Values represent the mean ± standard deviation of 3 independent experiments. **P<0.01, ***P<0.001.