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. 2022 Aug 22;16:954807. doi: 10.3389/fncel.2022.954807

FIGURE 3.

FIGURE 3

Lead-induced cellular alteration in offspring mouse cerebellum. (A) Representative images of TUNEL assay. Male (upper), female (lower). Scale bars, 50 μm. Positive control (DNase I) (B,C) Statistic analysis of TUNEL+ count. Any significant change was not shown in TUNEL assay. N = 3 per group. Data are representative at least three independently performed experiments and analyzed with one-way ANOVA, expressed as mean ± SEM. (D,E) Immunofluorescence images of BrdU (green) to identify differentiated cells. Scale bars, 50 μm. (F,G) Statistic analysis of BrdU+ count in cerebellum. Data are representative at least three independently performed experiments and analyzed with one-way ANOVA, expressed as mean ± SEM. (F) Result of BrdU immunostaining in male offspring. P group was significantly increased. N=3 per group, ***p < 0.001. (G) Result of BrdU immunostaining in female offspring. P group was significantly increased same as male offspring, but P + L was decreased compared to P group. N = 3 pergroups, *p < 0.05.