FIG. 1.
Effect of the presence of sotA and sotB on the expression of genes cloned into plasmid pACT3. E. coli NM522 cells containing plasmids pACTS1 (pelBsp-outS) (A), pABSC3 (pelBsp-outB) (B), pABTC2 (outB) (C), and pACTPB (pelB) (D) were transformed with plasmids pUC18, pUCS1 (sotA), and pUCS6 (sotB). Cells were grown for 3 h in the absence or presence of 10−4 M IPTG. Aliquots were loaded onto SDS-PAGE gel and immunoblotted with anti-OutS (A), anti-OutB (B and C), and anti-PelB (D) antibodies.