TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. chrysanthemi | ||
| 3937 | Wild type | Laboratory collection |
| A350 | lmrT(Con) lacZ | 15 |
| A2507 | lmrT(Con) lacZ crp::Cm | 29 |
| A3145 | lmrT(Con) lacZ sotA::uidA-kan | This work |
| A3501 | lmrT(Con) lacZ sotB::uidA-kan | This work |
| E. coli | ||
| NM522 | supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 rK− mK− F′ proAB lacIq ΔlacZM15 | Stratagene |
| P4X | Hfr metB relA1 spoT1 | Laboratory collection |
| NA1 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR uxaB::Mud(Aprlac) | 14 |
| 1701 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR (rbs-lac)op6.16 | J. Beckwith |
| 9665 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR trp::(malK-lac)1 | 1 |
| Plasmids | ||
| pACT3 | Cmr Ptac ori p15A | 9 |
| pEXT20 | Apr Ptac | 9 |
| pACTPB | pACT3 expressing pelB | This work |
| pABSC3 | pACT3 expressing pelBsp-outB | G. Condemine and V. E. Shevchik |
| pABCT2 | pACT3 expressing outB | G. Condemine and V. E. Shevchik |
| pACTS1 | pACT3 expressing pelBsp-outS | 32 |
| pBAD18 | Apr PBAD | 12 |
| pBADuid | uidA-kan cloned in the pBAD18 polylinker | This work |
| pBR322 | Tetr Apr | 4 |
| pBRS1 | pBR322 expressing sotA | This work |
| pBRS6 | pBR322 expressing sotB | This work |
| pSU9 | Cmr | 2 |
| pS1A | pSU9 expressing sotA | This work |
| pS6E | pSU9 expressing sotB | This work |
| pUC18 | Apr | Biolabs |
| pUCS1 | pUC18 expressing sotA | This work |
| pUCS6 | pUC18 expressing sotB | This work |
| pULB110 | Tetr Apr (Mu 3A) | 34 |