TABLE 2.
Expression of sotA-uidA and sotB-uidA fusions in the presence of various sugars
| Growth conditiona | Additional mutation | β-Glucuronidase activityb
|
|
|---|---|---|---|
| sotA-uidA | sotB-uidA | ||
| Glycerol | —c | 141 ± 5 | 16 ± 2 |
| Glycerol + lactose | — | 135 ± 7 | 18 ± 2 |
| Glycerol + IPTG | — | 141 ± 14 | 12 ± 2 |
| Glycerol + melibiose | — | 140 ± 9 | 49 ± 14 |
| Glycerol + arabinose | — | 104 ± 8 | 38 ± 12 |
| Glycerol + galacturonate | — | 216 ± 8 | 48 ± 6 |
| Glycerol + rhamnose | — | 147 ± 9 | 15 ± 2 |
| Glycerol + maltose | — | 152 ± 6 | 13 ± 1 |
| Glucose | — | 99 ± 7 | 62 ± 6 |
| Glucose | crp | 72 ± 6 | 125 ± 6 |
a
Bacteria were grown in M63 medium with 0.2% glycerol or glucose as the carbon source and 0.2% of the sugars tested or 1 mM IPTG.
b
β-Glucuronidase activity is expressed in nanomoles of p-nitrophenol formed per minute per milligram (dry weight) of bacteria. Each value is the average of four determinations.
c
—, none.