TABLE 2.
Distribution of proteins and hydrolytic activity in cells and culture medium of C. thermocellum grown on 0.5% cellobiose
| Fraction | Protein
|
Feruloyl esterase
|
Avicelase
|
Xylanase
|
||||
|---|---|---|---|---|---|---|---|---|
| mg | % | U | % | U | % | U | % | |
| Cell extracta | 0.81 | 39.7 | 0.005 | 2.1 | 0.001 | 2.4 | 0.49 | 5.3 |
| Culture mediuma | 1.23 | 60.3 | 0.238 | 97.9 | 0.04 | 97.6 | 8.72 | 94.7 |
| Culture medium after Avicel treatment | 0.98 | 48.0 | 0.002 | 0.8 | 0.004 | 9.8 | 1.56 | 16.9 |
| Purified cellulosome | 0.176 | 8.6 | 0.24 | 98.7 | 0.033 | 80.5 | 6.75 | 73.3 |
a
The sum of the activity in the cell extract and cultural medium was considered to be 100%.