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. 2022 Aug;11(8):1398–1407. doi: 10.21037/tp-22-410

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2022 Translational Pediatrics. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Experimental procedures and timeline. Experiment 1: Behavioral tests and ELISA to measure visceral pain threshold, the anxiety- and depression-like behaviors and neuroinflammation. Experiment 2: Testing the effects of EE on visceral pain, anxiety- and depression-like behaviors and neuroinflammation of NMS mice. PTT, pain tolerance test; SPT, sugar preference test; OFT, open field test; EE, enriched environment; ELISA, enzyme linked immunosorbent assay; NMS, neonatal maternal separation.