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. 2022 Jun 30;23(9):e54391. doi: 10.15252/embr.202154391

Figure EV4. Both HAT1 and NAT8 can bind to TRIM21.

Figure EV4

  • A
    IB analysis of WCLs and immunoprecipitates derived from 293T cells transfected with Flag‐HAT1 together with Flag‐TRIM21 constructs.
  • B
    IB analysis of WCLs and anti‐GST immunoprecipitated products derived from 293T cells transfected with indicated constructs.
  • C
    Purified Flag‐TRIM21 proteins were incubated with purified GST or GST‐NAT8 proteins as indicated for 90 min at 30°C, followed by pull down with anti‐GST antibody and Western blot analysis.
  • D
    IB analysis of WCLs and anti‐Flag immunoprecipitated products derived from 293T cells transfected with the indicated plasmids. 10 μM MG132 was added for 4 h before harvesting.

Data information: All IP and WB data in this work otherwise indicated are representative of at least three independent experiments.