Skip to main content
. 2022 Jun 30;23(9):e54391. doi: 10.15252/embr.202154391

Figure EV5. Effect of SIRT5 and TRIM21 mutual regulation on macrophage pro‐inflammatory cytokine production.

Figure EV5

  • A, B
    mRNA expression of SIRT5 (A) and TRIM21 (B) in LPS‐treated and untreated SIRT5 −/− BMDMs expressing Flag‐TRIM21 together with HA‐SIRT5 or various HA‐tagged SIRT5 mutants as indicated was determined by qRT‐PCR analysis and normalized to β‐actin. Macrophages were stimulation with 100 ng/ml LPS for 4 h.
  • C, D
    mRNA expression of SIRT5 (C) and TRIM21 (D) in LPS‐treated and untreated TRIM21 −/− BMDMs stably expressing HA‐SIRT5 together with Flag‐TRIM21 or various Flag‐tagged TRIM21 mutants as indicated was determined by qRT‐PCR analysis and normalized to β‐actin. Macrophages were stimulation with 100 ng/ml LPS for 4 h.
  • E, F
    mRNA expression of IFN‐β (E) and IL‐1β (F) in LPS‐treated and untreated BMDMs derived from wild‐type (WT), TRIM21 −/−, SIRT5 −/− and TRIM21 −/− SIRT5 −/− mice. Macrophages were stimulation with 100 ng/ml LPS for 4 h.

Data information: Data are means ± SD. In (A–F), n = 3 biological replicates. P‐values were determined by unpaired two‐tailed Student's t‐tests. ns, not significant.