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. 2022 Jul 8;23(9):e54677. doi: 10.15252/embr.202254677

Figure 2. IL‐21 increases cell cycle speed and rate of re‐entry.

Figure 2

  • A
    Experimental setup to study cell cycle progression of SWHEL B cells using BrdU injection.
  • B
    Flow cytometry analysis showing WT to Il21r −/− SWHEL B cell ratio of cells that have been in S phase and thus incorporated BrdU.
  • C
    Schematic depiction of BrdU and DNA content (7‐AAD) analysis by flow cytometry. At 4 h, BrdU‐positive cells with 2N DNA content mark those that have finished the cell cycle, whereas at 10 h 2N DNA content identifies cells that have not yet entered the subsequent cell cycle.
  • D
    Ratio of the proportion of BrdU‐positive WT and Il21r −/− SWHEL B cells with 2N DNA content 4‐, 10‐, and 12‐h post‐BrdU pulse on day 3.5 post‐immunization.

Data information: Data are representative of 5–7 biological replicates (n = 5–7) from two independent experiments. Statistical analysis by one‐sample t‐test. *P ≤ 0.05.