Figure 6. High‐ and low‐affinity B cell responses are amplified by IL‐21.

Experimental setup as in Fig 5A but analysis on day 7 post‐immunization and without CTV labeling of SW_HEL B cells.

• A–E
  Analysis of splenic SW_HEL B cells and PC by flow cytometry. (A) Proportion of SW_HEL B cells with a FAS⁺ GL7⁺ GC phenotype, (B) SW_HEL GC B cell count, and (C) representation of IL‐21R‐deficient cells among total SW_HEL GC B cells. (D) CD98⁺ CD138⁺ SW_HEL PC count and (E) representation of IL‐21R‐deficient cells among total SW_HEL PC.
• F
  Correlation of SW_HEL GC B cell and PC count.

Data information: Data are representative of 6–10 biological replicates (n = 6–10) from two independent experiments. Data in (B), (D), and (F) were analyzed after log transformation with statistical analysis by multiple paired t‐tests (A, B, D) and P‐values corrected for multiple comparisons using Holm–Šídák method or one‐way ANOVA with Tukey's post‐test (C) or t‐test (E). Analysis in (F) using Pearson correlation coefficient. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.