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. 2022 Jul 25;23(9):e54401. doi: 10.15252/embr.202154401

Figure 2. Light activation of optoYAP can activate downstream YAP target genes and control cell proliferation.

Figure 2

  • A
    Expression levels of ANKRD1, CTGF, and CYR61 transcripts in HEK293T cells transfected with optoYAP after 48 h of activation protocol. Gene expression level was normalised to the housekeeping gene, EIF1B. Horizontal bars represent mean and 95% confidence interval from six biological replicates across two independent experiments for each condition, ***P < 10−3 (unpaired t‐test).
  • B, C
    Cell proliferation assay. HEK293T (B) and HFF (C) cells were transfected with optoYAP and subjected to activation protocol as described in Fig 1A or kept in the dark for 1 week. Error bars are s.d., n = 3 independent experiments for each condition, **P < 10−2 (unpaired t‐test).