Figure EV3. LINC00839 mediates the acetylation of the NRF1 promoter by the Ruvb1/Tip60 complex to regulate OXPHOS, mitochondrial biogenesis, and CRC progression.

• A
  The pattern of 10 NRF1 promoter fragments (upper panel) and ChIP‐seq uniform peaks in the NRF1 promoter region was analyzed by ENCODE projects and visualized by UCSC Genome Browser (bottom panel).
• B–E
  The enrichment of the NRF1 promoter (P1‐P10) by Tip60 (B), Ruvb1 (C), H4K12ac (D), and H3K14ac (E) antibodies in LINC00839‐overexpressing and control LoVo cells as determined by CHIP assay. Normal IgG was used as a negative control.
• F, G
  ATP levels and NAD/NADH ratios in SW480 and LoVo cells were measured.
• H
  Expression of genes related to mitochondrial biogenesis and EMT in SW480 and LoVo cells as determined by WB.
• I
  The number of mitochondria in SW480 and LoVo cells was confirmed by TEM. Scale bars, 500 nm.
• J
  Invasion of SW480 and LoVo cells was assessed by Matrigel invasion assay. Scale bars, 100 μm.

Data information: In (B–G), the data are presented as the mean ± SD and were analyzed by Student's t‐test, n = 3 technical replicates. In (I, J), the data are presented as the mean ± SD and were analyzed by Student's t‐test, n = 3 biological replicates. Across experiments, the P‐values are as follows: ns = not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Source data are available online for this figure.