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. 2022 Jul 6;23(9):e52211. doi: 10.15252/embr.202052211

Figure EV1. ZIKV disrupts centrosome organization.

Figure EV1

  • A
    Mock and ZIKV‐infected H4 cells co‐stained for Centrin (green) and flavivirus envelope (red) to mark centrioles and infected cells, respectively. All cells were fixed 16 hpi.
  • B
    Twenty‐four hours post infection mock and ZIKV‐infected U87 cells co‐stained for Centrin (red) and NS3 (green). Boxed area represents a magnification of the boxed region.
  • C
    Mock and ZIKV‐infected U87 cells co‐stained for NS3 (green) and ER marker Calreticulin (red) 24 hpi.
  • D
    Quantification of mean fluorescence intensities ± SD of γ‐tubulin, CP110, and CEP164 in mock and ZIKV‐infected U87 cells. For fluorescence quantifications, eight cells were analyzed per experiment (n = 3). Asterisk denotes P < 0.005 (Student's t‐test).
  • E
    Immunoblot of mock or ZIKV‐infected U87 cell lysates probed for NS3, CP110, or γ‐tubulin. Actin served as a loading control.
  • F
    Immunofluorescence analysis of mock and ZIKV‐infected U87 cells co‐stained for Centrin (red) and CEP164 (green).
  • G
    Mock and ZIKV‐infected U87 cells were co‐stained for Centrin (green) and CEP90 (red) 16 hpi.

Data information: Scale bars indicate 5 μm for all images.