Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models
E14TG2a mESC (Mus musculus)	ATCC	CRL‐1821
HEK293T (Homo sapiens)	ATCC	CRL‐3216
Recombinant DNA
psiCHECK2‐mMov10‐3'UTR (WT) (mMov10 3'UTR)	Addgene this study	Cat # 178905
psiCHECK2‐mMOV103'UTR‐MRE16‐mut1	Addgene this study	Cat # 178906
psiCHECK2‐mMOV103'UTR‐MRE153‐mut4	Addgene this study	Cat # 178909
psiCHECK2‐mMOV103'UTR‐MRE16+MRE153‐mut	Addgene this study	Cat # 178910
pCDNA3‐T11HA‐hMOV10‐WT (human Mov10 cDNA)	Addgene this study	Cat # 178907
T10‐T7‐hMOV10 (human Mov10 cDNA)	Addgene this study	Cat # 185052
T11‐HA‐hORF1 (human L1 ORF1p cDNA)	Addgene this study	Cat # 185053
pCEP‐L1SM‐WT (hygro) (mouse L1 neoR cassette)	Maclennan et al (2017)
JJ‐L1SM WT (mouse L1 BlastR cassette)	Maclennan et al (2017)
JJ‐L1SM N21A (mouse nutant L1 BlastR cassette)	Maclennan et al (2017)
pKLV‐U6gRNA(BbsI)‐PGKpuro2ABFP‐L1mono3	Addgene this study	Cat # 73542
pKLV‐U6gRNA(BbsI)‐PGKpuro2ABFP‐L1mono1	Addgene this study	Cat # 73543
Antibodies
rabbit polyclonal anti‐ORF1p (IF 1:1,000, WB 1:4,000)	kind gift from Prof. O'Carroll	NA
mouse monoclonal 15C1BB anti‐MOV10 (IF 1:500)	Bethyl Laboratories Inc	A500‐009A‐T
rabbit polyclonal anti‐G3BP1 (IF 1:500)	Bethyl Laboratories Inc	A302‐033A
rabbit polyclonal anti‐LC3B antibody (IF 1:250)	Cell Signaling Technology	2775
rabbit polyclonal anti‐DDX6 (IF 1:500)	GeneTex	GTX102795
rat monoclonal anti‐HA (IF 1:500)	Roche	3F10
Rabbit monoclonal T7‐Tag (D9E1X) XP (IF 1:250)	Cell Signaling Technology	13246
Goat anti‐RENT1 antibody (IF 1:250)	Bethyl laboratories	A300‐038A
Alexa fluor 488 goat anti‐rat IgG (IF 1:4,000)	Life Technologies	11006
Alexa fluor 488 donkey anti‐mouse IgG (IF 1:4,000)	Life Technologies	A21202
Alexa fluor 546 donkey anti‐rabbit IgG (IF 1:4,000)	Life Technologies	A10040
Alexa fluor 647 donkey anti‐mouse IgG (IF 1:4,000)	Life Technologies	A31571
Alexa fluor 488 donkey anti‐goat IgG (IF 1:4,000)	Life Technologies	A11055
rabbit anti MOV10 antibody (WB 1:2,000)	Proteintech	10370‐1‐AP
rabbit polyclonal anti‐Dicer (WB 1:2,000)	Sigma	SAB42000087
rabbit polyclonal anti‐Argonaute2 (WB 1:2,000)	Cell Signaling Technologies	C34C6
rabbit anti‐Drosha (WB 1:2,000)	Cell Signaling Technologies	D28B1
mouse anti‐Tubulin antibody (WB 1:10,000)	GenScript	A01410
rabbit anti‐LaminB1 (WB 1:5,000)	Abcam	ab16048
anti‐rabbit IgG HRP‐linked (WB 1:10,000)	Cell Signaling Technologies	7076
anti‐rat IgG HRP‐linked antibody (WB 1:10,000)	Cell Signaling Technologies	7077
Oligonucleotides and sequence‐based reagents
Experiment	Fwd Primer	Rev Primer
mMOV10 3'UTR PCR amplification	taggcgatcgctcgaggccacagccgcccgcctt	ttgcggccagcggccttttgcatagaacagcattttgt
miR16‐5p (CTG>AGT) mut1 mutageneis	acccaagagtctaaaactcggaggaaggggg	tttagactcttgggttgtcttccctagc
miR153‐3p (TGC>CAT) mut4/mut1+mut4 mutagenesis	tgttctacataaaaggccgctggccgca	cttttatgtagaacagcattttgtttttctt
hMOV10 cDNA PCR amplification	ggtcggaggcggatccatgcccagtaagttcagctgc	gatatctgcagaattctcagagctcattcctccactc
hORF1 cDNA PCR amplification	ggtcggaggcggatccatggggaaaaaacagaac	gatatctgcagaattctcattacattttggcatgattttgc
Guide RNA L1UP mono3	caccgccagagaacctgacagcttc
Guide RNA L1UP mono1	caccgccagaggacaggtgcccgcc
mmu‐miR‐16‐5p mimic	uagcagcacguaaauauuggcg
mmu‐miR‐30e‐5p mimic	uguaaacauccuugacuggaag
mmu‐miR‐138‐5p mimic	agcugguguugugaaucaggccg
mmu‐miR‐153‐3p mimic	uugcauuagucacaaaagugauc
miRIDIAN microRNA negative control 1	catalog no CN‐001000‐01‐05
qPCR Rrm2	ccgagctggaaagtaaagcg	atgggaaagacaacgaagcg
qPCR Mov10	gacgatttacaaccacgacttca	gccagatttgcgatcttcattcc
qPCR Dicer	ccgatgatgcagcctctaatag	tccatctcgagcaattctctca
qPCR L1‐Tf	cagcggtcgccatcttg	caccctctcacctgttcagactaa
qPCR L1‐A	ggattccacacgtgatcctaa	tcctctatgagcagacctgga
qPCR L1‐Gf	ctccttggctccgggact	caggaaggtggccggttgt
qPCR L1‐ORF1	actcaaagcgaggcaacact	ctttgattgttgtgccgatg
qPCR L1‐ORF2	ggagggacatttcattctcatca	gctgctcttgtatttggagcataga
NB L1specifc	gagtttttgagtctgtatcc	ctctccttagtttcagtgg
Chemicals, enzymes and other reagents
Fibronectin	Sigma	FC010
Puromycin	Sigma	P8833
Hygromycin	Invitrogen	10687010
G418	Invitrogen	10131035
Blasticidin	Invitrogen	R21001
DMEM Media	Sigma	D6429‐500ML
Penicillin/Streptomycin	Sigma	P0781‐100ML
0.05% Trypsin‐EDTA	Life Technologies	25300054
PBS1X	Life Technologies	10010015
2‐ß‐mercaptoethanol	Life Technologies	31350010
FBS	Life Technologies	10270‐106
Lipofectamine™ RNAiMAX Transfection Reagent	Life Technologies	13778030
Lipofectamine™ 2000 Transfection Reagent	Life Technologies	11668019
Lipofectamine™ 3000 Transfection Reagent	Life Technologies	L3000015
1,6‐Hexanediol	Sigma	H11807
Sodium Arsenite	Sigma	S7400
Tris(carboxyethyl)phosphine	Sigma	68957
Tris‐HCl	AppliChem	A2937
NaCl	Merck	1.06404.1000
MgCl2	Sigma	M8266
IGEPAL CA‐630	Sigma	I3021‐50ML
Sodium dodecyl sulfate	Sigma	L6026
Protease inhibitor cocktails	Roche	5892791001
Tween20	Sigma	P1379
Coomassie	VWR	443283M
Triton‐X	Sigma	93443
PIPES	Sigma	80635
NotI‐HF	NEB	R3189S
BamH1‐HF	NEB	R3136
XhoI‐HF	NEB	R0146
EcoRI‐HF	NEB	R0101
Bbs1	NEB	R0539
In‐Fusion® Snap Assembly Master Mix	Takara	638944
CloneAmp HiFi PCR Premix	Takara	639298
Vectashield	Vector Laboratories	H‐1000
DAPI	Sigma	D9542
Trizol	Life Technologies	15596018
Nick translation kit	Abbot Molecular	06J40‐020
Red‐dUTP	Enzo Life sciences	ENZ 42854
Ribonucleoside Vanadyl Complex	NEB	S1420S
Trisodium citrate	Sigma	S4641
DNA Clean & Concentrator‐25	Zymo Research	D4033
Clarify™ Western ECL substrate	BioRad	1705060
SuperSignal™ West Femto Maximum Sensitivity Substrate	Therno Scientific	34094
RQ1 Rnase‐Free DNase kit	Promega	M6101
GoScript™ Reverse Transcriptase Kit	Promega	A5001
KAPA SYBR® FAST qPCR Kit Optimized for Light Cycler® 480	KAPA Biosystems, Sigma	KK4610
PerfectHybTM Plus	Sigma	H7033
Dual‐Glow Luciferase Assay kit	Promega	E2920
Crystal Violet	Sigma	1014080025
Software
Prism 9.2.0	GraphPad	https://www.graphpad.com
Fiji	Fiji ImageJ	Nature Methods, 9(7), 676–682
Microsoft Excel	Microsoft
ImageLab v6.1.0	Bio‐Rad Laboratories	https://www.bio‐rad.com
Other
GloMax® Discover Multimode Microplate Reader	Promega
Roche Light Cycler 480	Roche