Figure 6. TMEM175 regulates apoptosis.

• A
  Wild‐type (WT) or TMEM175 KO HEK293T cells were treated with vehicle or 2 mM MPP⁺ (1‐methyl‐4‐phenylpyridinium) for 24 h. Apoptotic cell death was detected by Annexin V–fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining followed by flow cytometric analysis.
• B
  Statistics of the apoptosis rates in (A). Four independent experiments were performed.
• C
  Apoptosis detection of HEK293T cells transfected with empty vectors or nontagged TMEM175 treated with vehicle or 2 mM MPP⁺.
• D
  Statistics of the apoptosis rates in (C). Three independent experiments were performed.
• E
  Apoptosis detection of vector, TMEM175, TMEM175‐V145A, or TMEM175‐R377V‐transfected HEK293T cells.
• F
  Statistics of the apoptosis rates in (E). Four independent experiments were performed.

Data information: The data are presented as the mean ± SEM. Statistical significance was analyzed with two‐sided Student's t‐tests, and is indicated with NS for not significant (P > 0.05), * for P < 0.05, ** for P < 0.01, and *** for P < 0.001.