Figure 1. P4‐P30 rapamycin treatment increases mouse lifespan.

• A
  Schematic illustration of the experimental procedure and results. Control mice were intraperitoneally injected daily with EtOH from postnatal day 4 to postnatal day 30 (P4‐P30). Treated mice were intraperitoneally injected daily with rapamycin during two distinct temporal windows, P4‐P30 or P30‐P60. P4‐P30 rapamycin‐treated mice show a lifespan increment compared with control and P30‐P60 rapamycin‐treated mice.
• B
  Survival curves of control mice, P4‐P30 rapamycin‐treated mice, and P30‐P60 rapamycin‐treated mice including data from both sexes (males + females). Log‐rank (Mantel–Cox) test.
• C, D
  Survival curves of male (C) and female (D) control mice, P4‐P30 rapamycin‐treated mice, and P30‐P60 rapamycin‐treated mice. Log‐rank (Mantel–Cox) test.
• E
  Log‐rank test on survival analysis (summary table).