Figure EV4. Early‐transient 1 μM and 50 μM rapamycin treatment does not induce a time‐dependent effect on lifespan.

• A
  Schematic illustration of the experimental procedure and results. Flies were transiently treated during larval stages from 72 h after egg laying to puparium formation with 1 μM (gray) or 50 μM rapamycin (yellow).
• B
  Survival curves of w ^iso31 flies transiently treated from 72 h after egg laying until puparium formation (males + females) with EtOH (control) or rapamycin 1 μM.
• C, D
  Survival curves of male (C) and female (D) w ^iso31 flies transiently treated from 72 h after egg laying until puparium formation with EtOH (control) or rapamycin 1 μM.
• E
  Survival curves of w ^iso31 flies transiently treated from 72 h after egg laying until puparium formation (males + females) with EtOH (control) or rapamycin 50 μM.
• F, G
  Survival curves of male (F) and female (G) w ^iso31 flies transiently treated from 72 h after egg laying until puparium formation with EtOH (control) or rapamycin 50 μM.

Data information: Log‐rank (Mantel–Cox) test; n.s., not significant.