Figure 6. Early‐transient rapamycin treatment on w ^iso31 Drosophila melanogaster induces a time‐dependent effect on lifespan.

• A
  Schematic illustration of the experimental procedure and results. Flies were transiently treated during larval stages with rapamycin 200 μM starting from 72 h after egg laying to puparium formation (red) or during adulthood, from day 0 to day 10 (orange), from day 10 to day 13 (blue), or from day 10 to day 20 (purple). Rapamycin administration during development and during the first 10 days of life, but not at later time points, leads to lifespan increment.
• B
  Survival curves of w ^iso31 flies transiently treated from 72 h after egg laying till puparium formation (males + females) with EtOH (control) or rapamycin 200 μM.
• C, D
  Survival curves of male (C) and female (D) w ^iso31 flies transiently treated from 72 h after egg‐laying till puparium formation with EtOH (control) or rapamycin 200 μM.
• E
  Survival curves of w ^iso31 flies transiently treated in adult stage, from day 10 to 13 (males + females), with EtOH (control) or rapamycin 200 μM.
• F, G
  Survival curves of male (F) and female (G) w ^iso31 flies transiently treated in adult stage, from day 10 to 13, with EtOH (control) or rapamycin 200 μM.
• H
  Survival curves of w ^iso31 flies transiently treated from day 0 to day 10 (males + females) with EtOH (control) or rapamycin 200 μM.
• I, J
  Survival curves of male (I) and female (J) w ^iso31 flies transiently treated from day 0 to day 10 with EtOH (control) or rapamycin 200 μM.
• K
  Survival curves of w ^iso31 flies transiently treated from day 10 to 20 (males + females), with EtOH (control) or rapamycin 200 μM.
• L, M
  Survival curves of male (L) and female (M) w ^iso31 flies transiently treated from day 10 to 20, with EtOH (control) or rapamycin 200 μM.

Data information: Log‐rank (Mantel–Cox) test. ***P < 0.0005, n.s., not significant.