Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 7;23(9):e55299. doi: 10.15252/embr.202255299

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Authors. Published under the terms of the CC BY 4.0 license.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

A
Schematic illustration of the experimental procedure and results. Constitutive dST1 overexpression during the entire life (blue) of flies does not increase lifespan. Transient dST1 overexpression during larval stage (red) leads to lifespan increment.

B
Survival curves of flies harboring constitutive dST1 upregulation (TubGal4/UAS‐dST1) compared with control strain (UAS‐GFP;TubGal4/+) males + females. Log‐rank (Mantel–Cox) test, ***P < 0.0005.

C, D
Survival curves of flies harboring constitutive dST1 upregulation (TubGal4/UAS‐dST1) in male (C) and female (D) compared with control strain (UAS‐GFP;TubGal4/+). Log‐rank (Mantel–Cox) test, ***P < 0.0005.

E
Survival curves of flies upon upregulation of dST1 during larval stages (tubGal80 ^TS/+;TubGal4/UAS‐dST1) compared with the control strain tubGal80 ^TS/UAS‐GFP;TubGal4/+ (males + females) and the parental control tubGal80 ^TS ;TubGal4 (males + females). Log‐rank (Mantel–Cox) test, ***P < 0.0005.

F, G
Survival curves of male (F) and female (G) flies upon upregulation of dST1 during larval stages in (tubGal80 ^TS/+;TubGal4/UAS‐dST1) compared to control strain tubGal80 ^TS/UAS‐GFP;TubGal4/+ and the parental control tubGal80 ^TS ;TubGal4. Log‐rank (Mantel–Cox) test, ***P < 0.0005, n.s., not significant.

H
Climbing performance index of tubGal80 ^TS/UAS‐GFP;TubGal4/+ and tubGal80 ^TS/+;TubGal4/UAS‐dST1 at different time points: 40 days (n = 176 and 196, respectively; biological replicates), 60 days (n = 159 and 178, respectively; biological replicates), and 80 days (n = 119 and 158, respectively; biological replicates). Upregulation of dST1 has been induced only during larval stages. Data are indicated as mean + SEM. Two‐way ANOVA, ***P < 0.0005.

I
Climbing performance index of male tubGal80 ^TS/UAS‐GFP;TubGal4/+ and tubGal80 ^TS/+;TubGal4/UAS‐dST1 at different time points: 40 days (n = 93 and 93, respectively; biological replicates), 60 days (n = 79 and 81, respectively; biological replicates), and 80 days (n = 41 and 70, respectively; biological replicates). Upregulation of dST1 has been induced only during larval stages. Data are indicated as mean + SEM. Two‐way ANOVA, ***P < 0.0005.

J
Climbing performance index of female tubGal80 ^TS/UAS‐GFP;TubGal4/+ and tubGal80 ^TS/+;TubGal4/UAS‐dST1 at different time points: 40 days (n = 83 and 103, respectively; biological replicates), 60 days (n = 80 and 97, respectively; biological replicates), and 80 days (n = 78 and 88, respectively; biological replicates). Upregulation of dST1 has been induced only during larval stages. Data are indicated as mean + SEM. Two‐way ANOVA; **P < 0.005, ***P < 0.0005.