Table 3.

Sperm analysis and sperm abnormalities of rats following exposure to AFB₁ for 28 days.

*Total rats per grouping	Control (6)	AFB1 (6)	AFB1 + SBE-05-D1 (6)	AFB1 + SBE-05-D2 (6)	AFB1 + SBE-06-D1 (6)	AFB1 + SBE-06-D2 (6)	AFB1 + SBE-07-D1 (6)	AFB1 + SBE-07-D2 (6)
Sperm functional analysis
Motility	93.00 ± 2.74	48.33 ± 2.58*	65.00 ± 5.48 #	67.50 ± 5.00 #	68.33 ± 4.08 #	62.00 ± 4.47 #	61.67 ± 4.08 #	56.67 ± 5.16
Viability	95.80 ± 3.49	92.50 ± 2.74	96.50 ± 1.64	96.50 ± 1.72	96.50 ± 1.64 #	96.80 ± 1.64	96.50 ± 1.64	96.50 ± 1.64
Testicular sperm count	5.18 ± 0.04	4.92 ± 0.04*	5.18 ± 0.04 #	5.17 ± 0.05 #	5.16 ± 0.05 #	5.18 ± 0.04 #	5.16 ± 0.05 #	5.16 ± 0.05 #
Epididymal sperm count	116.60 ± 2.88	86.20 ± 8.14*	98.83 ± 10.53	99.75 ± 8.88	99.17 ± 6.94	89.60 ± 11.80	92.83 ± 7.08	123.20 ± 5.31 #
Sperm abnormalities
Abnormality of the head (%)	2.22 ± 0.30	2.43 ± 0.48	2.30 ± 0.29	2.53 ± 0.45	2.27 ± 0.53	2.14 ± 0.40	2.35 ± 0.41	2.18 ± 0.29
Abnormality of the mid-piece (%)	4.10 ± 0.39	6.20 ± 0.35*	5.31 ± 0.39 #	5.28 ± 0.41	5.41 ± 0.43	5.16 ± 0.18 #	5.41 ± 0.35	5.65 ± 0.40
Abnormality of the tail (%)	5.23 ± 0.49	6.67 ± 0.39*	6.25 ± 0.53	6.25 ± 0.73	6.08 ± 0.27	5.97 ± 0.63	6.33 ± 0.52	6.36 ± 0.36
Total abnormality (%)	116.60 ± 2.88	123.20 ± 5.30	98.83 ± 10.53	99.75 ± 8.88	99.17 ± 6.94	89.60 ± 11.80	92.83 ± 7.08	88.17 ± 8.72

AFB₁: aflatoxin B₁; SBE: Sorghum bicolor extract.

AFB₁, 50 µg/kg; AFB₁ + SBE-05-D1, 0.05 + 5 mg/kg; AFB₁ + SBE-5-D2, 0.05 + 10 mg/kg; AFB₁ + SBE-06-D1, 0.05 + 5 mg/kg; AFB₁ + SBE-06-D2, 0.05 + 10 mg/kg; AFB₁ + SBE-07-D1, 0.05 + 5 mg/kg; and AFB₁ + SBE-07-D2, 0.05 + 10 mg/kg. Values are expressed as mean ± SD for six rats per group.

^*Values differ significantly from control (p < 0.05).

^#Values differ significantly from AFB₁ alone (p < 0.05).