Figure S3.

There are 12 S/T-P motifs in D. melanogaster Ana2. (A) Schematic illustrates the position and conservation of the S/T-P motifs in D. melanogaster Ana2 and indicates which of these have been shown to be phosphorylated by either Cdk/Cyclin B (this study) or a recombinant Plk4 kinase domain (Dzhindzhev et al., 2017) in vitro, or have been shown to be phosphorylated in either embryo (Dzhindzhev et al., 2017) or S2 cell extracts (McLamarrah et al., 2018) by MS. (B) A multiple sequence alignment (MSA) showing the conservation of S/T-P motifs (highlighted in red) in Ana2 from 15 different Drosophila species. Note that the numbering of the MSA in (B) does not precisely line up with the numbering in the schematic (A) due to the introduction of gaps in the D. melanogaster sequence shown in the MSA (B).