FIG. 1.

Western blot analysis of whole cell lysates prepared from M. catarrhalis strains 012E and TTA37 and their corresponding isogenic mutants. (A) Wild-type M. catarrhalis 012E (lanes 1 and 2), the uspA1 mutant 012E.1 (lanes 3 and 4), the uspA2 mutant 012E.2 (lanes 5 and 6), and the uspA1 uspA2 double mutant 012E.12 (lanes 7 and 8). (B) Wild-type M. catarrhalis TTA37 (lanes 1 and 2), the uspA1 mutant TTA37.1 (lanes 3 and 4), the uspA2H mutant TTA37.2 (lanes 5 and 6), and the uspA1 uspA2H double mutant TTA37.12 (lanes 7 and 8). The UspA1- and UspA2-reactive MAb 17C7 was used to probe lanes 1, 3, 5, and 7 in both panels; the UspA1-specific MAb 24B5 was used to probe lanes 2, 4, 6, and 8 in both panels. Molecular mass markers are shown to the left in kilodaltons.